Journal: Frontiers in Plant Science

Article Title: Identification of Metal Stresses in Arabidopsis thaliana Using Hyperspectral Reflectance Imaging

doi: 10.3389/fpls.2021.624656

Figure Lengend Snippet: Chlorophyll spectral components of A. thaliana under different stress conditions from MCR analysis of hyperspectral reflectance data. (A) chlorophyll component spectra, (B) chl-1 (green) and chl-2 (blue) spectra in plant images under control and stress conditions, and (C,D) box and whisker plot of the mean signal intensity for the chl-1 (C) and chl-2 (D) components under control and stress conditions after 9 days of treatment. Mean signal intensity of the chlorophyll stress components is the average intensity of all plant pixels in an image for at least five biological replicates. For each box plot, the top and bottom of the box corresponds to the 25th and 75th percentile of the data, respectively, while the red line in the middle of the notch corresponds to the sample median across the replicates. The whiskers above and below each box show the extent of the data, aside from any outliers (marked with red asterisks). Observations are defined as outliers if they are more than 1.5 times the interquartile range away from the top or bottom of the box. Results of the Games-Howell test for statistical significance are shown in .

Article Snippet: Preprocessing of hyperspectral reflectance data was performed by reading hyperspectral data files (reflectance data normalized to the white reference spectra) from the Headwall Photonics Hyperspec ® VNIR E-Series hyperspectral imager into a custom software application, executing an empirically derived algorithm on the reflectance data, and outputting a comma separated value file representing a plant mask of the hyperspectral reflectance data.

Techniques: Control, Whisker Assay

Journal: Frontiers in Plant Science

Article Title: Identification of Metal Stresses in Arabidopsis thaliana Using Hyperspectral Reflectance Imaging

doi: 10.3389/fpls.2021.624656

Figure Lengend Snippet: Stress spectral components of A. thaliana under different stress conditions from MCR analysis of hyperspectral reflectance data. (A) stress component spectra, (B) CuCl 2 (green), NaCl (blue), and CsCl-2 (red) stress spectra in plant images under control and stress conditions, and (C–F) box and whisker plot of the mean signal intensity for the stress spectral components of NaCl (C) , CuCl 2 (D) , CsCl-1 (E) , and CsCl-2 (F) under control and stress conditions after 9 days of treatment. Mean signal intensity of each stress component is the average intensity of all plant pixels in an image for at least five biological replicates. For each box plot, the top and bottom of the box corresponds to the 25th and 75th percentile of the data, respectively, while the red line in the middle of the notch corresponds to the sample median across the replicates. The whiskers above and below each box show the extent of the data, aside from any outliers (marked with red asterisks). Observations are defined as outliers if they are more than 1.5 times the interquartile range away from the top or bottom of the box. Results of the Games-Howell test for statistical significance are shown in .

Article Snippet: Preprocessing of hyperspectral reflectance data was performed by reading hyperspectral data files (reflectance data normalized to the white reference spectra) from the Headwall Photonics Hyperspec ® VNIR E-Series hyperspectral imager into a custom software application, executing an empirically derived algorithm on the reflectance data, and outputting a comma separated value file representing a plant mask of the hyperspectral reflectance data.

Techniques: Control, Whisker Assay